Difference between revisions of "Part:BBa K914006:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K914006 short</partinfo> | <partinfo>BBa_K914006 short</partinfo> | ||
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It has very hight efficiency. | It has very hight efficiency. | ||
+ | ===Sequence=== | ||
+ | The sequence available here was automatically generated by the Parts Registry as a composite part assembled from several basic parts. In fact, during the ligation, one of the created scars is slightly different: the actual sequence of the scar between the RBS and the I-SceI gene is TACTAGAG (and not TACTAG) | ||
Revision as of 13:23, 21 September 2012
I-SceI meganuclease
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
It has very hight efficiency.
Sequence
The sequence available here was automatically generated by the Parts Registry as a composite part assembled from several basic parts. In fact, during the ligation, one of the created scars is slightly different: the actual sequence of the scar between the RBS and the I-SceI gene is TACTAGAG (and not TACTAG)
Source
Meganuclease was amplified from the chromosome of SMR6316 E.Coli strain (Dr. Rosenberg).