Difference between revisions of "Part:BBa K777001"
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[[Image:Table_K777101-K777108_new.jpg|thumb|240px|right|'''Fig. 1:''' Overview of BioBricks containing the ''Tar'' gene downstream of Anderson promoters. (Activity according to Berkeley 2006)]] | [[Image:Table_K777101-K777108_new.jpg|thumb|240px|right|'''Fig. 1:''' Overview of BioBricks containing the ''Tar'' gene downstream of Anderson promoters. (Activity according to Berkeley 2006)]] | ||
| − | The transmembrane chemoreceptor Tar of <i>E. coli</i> mediates chemotaxis towards aspartate. | + | The transmembrane chemoreceptor Tar of <i>E. coli</i> mediates chemotaxis towards aspartate and exists as a functional homodimer. |
Here we used a set of 8 constitutive [https://parts.igem.org/Part:BBa_J23100 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''Tar'' expression affect the chemotaxis of ''E. coli''. | Here we used a set of 8 constitutive [https://parts.igem.org/Part:BBa_J23100 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''Tar'' expression affect the chemotaxis of ''E. coli''. | ||
Revision as of 09:40, 21 September 2012
Tar receptor under the control of constitutive promoter J23100
The transmembrane chemoreceptor Tar of E. coli mediates chemotaxis towards aspartate and exists as a functional homodimer.
Here we used a set of 8 constitutive Anderson promoters from the 2006 Berkeley group to test how different levels of constitutive Tar expression affect the chemotaxis of E. coli.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1323
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 152