Difference between revisions of "Part:BBa K861100:Experience"
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<br/><li>Sense CGAATTCTTCTAGAGATGAGTATCCTGACCCGGTGG | <br/><li>Sense CGAATTCTTCTAGAGATGAGTATCCTGACCCGGTGG | ||
<br/>After amplifying by PCR, the DNA fragmentation was examined by agarose gel electrophoresis, the lads show that the sizes of the gene were correct. | <br/>After amplifying by PCR, the DNA fragmentation was examined by agarose gel electrophoresis, the lads show that the sizes of the gene were correct. | ||
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<img src="https://static.igem.org/mediawiki/2012/4/42/BcsA.tif" width="100" height="400" hspace="2" vspace="1" border="2" align="CENTER"/> | <img src="https://static.igem.org/mediawiki/2012/4/42/BcsA.tif" width="100" height="400" hspace="2" vspace="1" border="2" align="CENTER"/> | ||
</CENTER> | </CENTER> | ||
Latest revision as of 03:49, 21 September 2012
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Applications of BBa_K861100
As for the genes we clone, there is no difference between E. coli str. K12 MG1655 and more available DH5α. we purified and amplified these genes from genome of Escherichia coli str. DH5α using PCR. The primers contain the standard restriction enzyme cutting sites. The sequences of the primers used are as below.
After amplifying by PCR, the DNA fragmentation was examined by agarose gel electrophoresis, the lads show that the sizes of the gene were correct.
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UNIQfc27e9a730ed1d73-partinfo-00000001-QINU UNIQfc27e9a730ed1d73-partinfo-00000002-QINU