Difference between revisions of "Part:BBa K743008:Design"

 
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<partinfo>BBa_K743008 short</partinfo>
 
<partinfo>BBa_K743008 short</partinfo>
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===Source===
 
===Source===
  
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Both [https://parts.igem.org/Part:BBa_K743001 BBa_K743001] and [https://parts.igem.org/Part:BBa_K743002 BBa_K743002] were PCR amplified from genomic dna. [https://parts.igem.org/Part:BBa_P1007 BBa_K743001] and [https://parts.igem.org/Part:BBa_B0015 BBa_B0015] were amplified from biobricks.
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Both [https://parts.igem.org/Part:BBa_K743001 BBa_K743001] and [https://parts.igem.org/Part:BBa_K743000 BBa_K743000] were PCR amplified from genomic dna. [https://parts.igem.org/Part:BBa_P1007 BBa_K743001] and [https://parts.igem.org/Part:BBa_B0015 BBa_B0015] were amplified from biobricks.
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Please note that [https://parts.igem.org/Part:BBa_K743001 BBa_K743001] has a EcoR1 restriction site at position 132, because of this this construct '''can not''' be handled as an upstream part.
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This also should be considered when making analytic digestions.
  
 
===References===
 
===References===

Latest revision as of 01:42, 21 September 2012

psb1C3_IntKR recombination plasmid for Synechocystis PCC6803


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1758
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1758
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1758
    Illegal XhoI site found at 2255
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1758
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1758
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 511


Design Notes

Using a double terminator followed by a reversed antibiotic resistance cds allows the use of the same terminator sequence for both the resistance casette and any desired coding sequence placed upstream


Source

Both BBa_K743001 and BBa_K743000 were PCR amplified from genomic dna. BBa_K743001 and BBa_B0015 were amplified from biobricks.

Please note that BBa_K743001 has a EcoR1 restriction site at position 132, because of this this construct can not be handled as an upstream part. This also should be considered when making analytic digestions.

References