Difference between revisions of "Part:BBa K743006"

Revision as of 23:16, 20 September 2012

psb1C3_IntK recombination plasmid for Synechocystis PCC6803

This plasmid allows the integration of a Kanamycin resistance casette followed by a double terminator in to Synechocystis chromosome, this allows for selection in this cyanobacteria. It has a Chloramphenicol resistance casette for selection in E.coli The use of BBa_K743000 and BBa_K743001 as recombination sites has no deleterious phenotypic effects on the cells. The plasmid is intended to be used as a starting backbone for further adition of dna parts by Gibson assembly. It was used by [http://2012.igem.org/Team:UC_Chile UC_Chile 2012 team] to succesfully transform Synechocystis

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 1774
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 1774
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 1774
Illegal XhoI site found at 2271
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 1774
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 1774
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 511

@@ Line 3: / Line 3: @@
 This plasmid allows the integration of a Kanamycin resistance casette followed by a double terminator in to Synechocystis chromosome, this allows for selection in this cyanobacteria. It has a Chloramphenicol resistance casette for selection in E.coli
-The use of BBa_K743000 and BBa_K743001 as recombination sites has no phenotypic effects on the cells.
+The use of [https://parts.igem.org/Part:BBa_K743000 BBa_K743000] and [https://parts.igem.org/Part:BBa_K743001 BBa_K743001] as recombination sites has no deleterious phenotypic effects on the cells.
 The plasmid is intended to be used as a starting backbone for further adition of dna parts by Gibson assembly.
 It was used by [http://2012.igem.org/Team:UC_Chile UC_Chile 2012 team] to succesfully transform Synechocystis