Difference between revisions of "Part:BBa K743000:Design"

Latest revision as of 22:05, 20 September 2012

Synechocystis PCC6803 neutral recombination site.

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 511

Design Notes

According to literature, in Synechocystis the recombination sites must be at least 450 bp long to allow a rasonably high transformation efficiency, being approximately 1200 bp the optimum size.

Source

PCR amplified from synechocystis PCC6803 chromosome, orf slr0370.

References

http://genome.kazusa.or.jp/cyanobase/Synechocystis

Kufryk, G. I., Sachet, M., Schmetterer, G., & Vermaas, W. F. J. (2002). Transformation of the cyanobacterium Synechocystis sp. PCC 6803 as a tool for genetic mapping: optimization of efficiency. FEMS microbiology letters, 206(2), 215-9.

Xu, Y., Alvey, R. M., Byrne, P. O., Graham, J. E., Shen, G., & Bryant, D. A. (2011). Photosynthesis Research Protocols. (R. Carpentier, Ed.)Methods, 684, 273-293.

Kucho, K.-ichi, Aoki, K., Itoh, S., & Ishiura, M. (2005). Improvement of the bioluminescence reporter system for real-time monitoring of circadian rhythms in the cyanobacterium Synechocystis sp. strain PCC 6803. Genes & genetic systems, 80(1), 19-23.

Revision as of 15:22, 20 September 2012 (view source) Juan Alamos (Talk \| contribs) (→‎Design Notes) ← Older edit		Latest revision as of 22:05, 20 September 2012 (view source) Juan Alamos (Talk \| contribs) (→‎References)
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	Xu, Y., Alvey, R. M., Byrne, P. O., Graham, J. E., Shen, G., & Bryant, D. A. (2011). Photosynthesis Research Protocols. (R. Carpentier, Ed.)Methods, 684, 273-293.		Xu, Y., Alvey, R. M., Byrne, P. O., Graham, J. E., Shen, G., & Bryant, D. A. (2011). Photosynthesis Research Protocols. (R. Carpentier, Ed.)Methods, 684, 273-293.
		+
		+	Kucho, K.-ichi, Aoki, K., Itoh, S., & Ishiura, M. (2005). Improvement of the bioluminescence reporter system for real-time monitoring of circadian rhythms in the cyanobacterium Synechocystis sp. strain PCC 6803. Genes & genetic systems, 80(1), 19-23.