Difference between revisions of "BBa K731700 and BBa K731710 measurements"
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IN VIVO
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'''''IN VIVO'' ANALYSIS'''
 
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CELLS GROWTH AND SAMPLES PREPARATION (starting from a glycerol stock):
 
CELLS GROWTH AND SAMPLES PREPARATION (starting from a glycerol stock):
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* Transcriptional terminator consisting of a 64 bp stem-loop.
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* 100ul of cells in 10 ml fresh LB (4 glycerol stocks of each platform with or without terminator in BL21(DE3) pLysS) in 50ml falcon.
  
 
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Measurements of part K731700 and K731710
 
Measurements of part K731700 and K731710

Revision as of 20:41, 20 September 2012

Hello world

In this page we are really proud to introduce you the protocol we (Giacomo and Anna) developed for the characterization transcriptional terminators effects on gene expression. (Tested on BBa_J64997)

The develop of this protocolcost us not only sleepless night but also sunny and beautiful weekend of trekking so just shut up and read it all.


IN VIVO ANALYSIS

  • 100ul of cells in 10 ml fresh LB (4 glycerol stocks of each platform with or without terminator in BL21(DE3) pLysS) in 50ml falcon.

CELLS GROWTH AND SAMPLES PREPARATION (starting from a glycerol stock):

Measurements of part K731700 and K731710