Difference between revisions of "Part:BBa K743004:Design"
Juan Alamos (Talk | contribs) (→Design Notes) |
Juan Alamos (Talk | contribs) (→Design Notes) |
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===Design Notes=== | ===Design Notes=== | ||
| − | Part K743001 contains a EcoR1 site in position 132. This shouldn´t represent a problem as long this composite part is digested as a downstream part (i.e with enzymes different from | + | Part K743001 contains a EcoR1 site in position 132. This shouldn´t represent a problem as long this composite part is digested as a downstream part (i.e with enzymes different from EcoR1) |
No mutation was done so that complete homology with Synechocystis chromosome is achieved | No mutation was done so that complete homology with Synechocystis chromosome is achieved | ||
Revision as of 19:40, 20 September 2012
psb1C3_IntK plasmid with RFP under psaAB promoter
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 2752
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2752
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2752
Illegal XhoI site found at 3249 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 2752
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 2752
Illegal AgeI site found at 1270
Illegal AgeI site found at 1382 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 511
Design Notes
Part K743001 contains a EcoR1 site in position 132. This shouldn´t represent a problem as long this composite part is digested as a downstream part (i.e with enzymes different from EcoR1) No mutation was done so that complete homology with Synechocystis chromosome is achieved
Source
BBa_B0015 terminator sequence was obtained from the registry and ligated with Synechocystis genomic region sll0337 by Gibson assembly