Difference between revisions of "Part:BBa K743001:Design"

 
(Design Notes)
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K743001 short</partinfo>
 
<partinfo>BBa_K743001 short</partinfo>
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According to literature, in Synechocystis the recombination sites must be at least 450 bp long to allow a rasonably high transformation efficiency, being approximately 1200 bp the optimum size.
 
According to literature, in Synechocystis the recombination sites must be at least 450 bp long to allow a rasonably high transformation efficiency, being approximately 1200 bp the optimum size.
  
 
+
This part has a EcoR1 site at position 132 wich wasn´t mutated so that there´s complete homology with Synechocystis chromosome region. The restricion site should´t be a problem for standard assemblies as long as this part is used as a downstream brick.
  
 
===Source===
 
===Source===

Latest revision as of 19:09, 20 September 2012

Synechocystis PCC6803 neutral recombination site.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 132
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 132
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 132
    Illegal XhoI site found at 629
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 132
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 132
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

According to literature, in Synechocystis the recombination sites must be at least 450 bp long to allow a rasonably high transformation efficiency, being approximately 1200 bp the optimum size.

This part has a EcoR1 site at position 132 wich wasn´t mutated so that there´s complete homology with Synechocystis chromosome region. The restricion site should´t be a problem for standard assemblies as long as this part is used as a downstream brick.

Source

PCR amplified from Synechocystis PCC6803 chromosome, orf slr0337

References