Difference between revisions of "Part:BBa K743000:Design"

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===References===
 
===References===
 +
http://genome.kazusa.or.jp/cyanobase/Synechocystis

Revision as of 14:53, 20 September 2012

Synechocystis PCC6803 neutral recombination site.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 511


Design Notes

According to literature, in Synechocystis the recombination sites must be at least 450bp long to allow a rasonably high transformation efficiency, being 700bp the optimum size.


Source

PCR amplified from synechocystis PCC6803 chromosome, orf slr0370.

References

http://genome.kazusa.or.jp/cyanobase/Synechocystis