Difference between revisions of "Part:BBa K759004"
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This gene codes the enzyme“Beta- ketothiolase (acetyl-CoA acetyltransferase)”which catalyzes the conversion of two acetyl-CoA molecules into acetoacetyl-CoA. It is an enzyme that catalyzes the first step of P3(HB) producing pathway like the picture below. | This gene codes the enzyme“Beta- ketothiolase (acetyl-CoA acetyltransferase)”which catalyzes the conversion of two acetyl-CoA molecules into acetoacetyl-CoA. It is an enzyme that catalyzes the first step of P3(HB) producing pathway like the picture below. | ||
| − | This gene was cloned from pGEM phbCAB | + | This gene was cloned from pGEM phbCAB which carries the synthetic operon genes (phaA,phaB,phaC) of Ralstonia eutropha. In the pGEM phbCAB phaA, we found a mutation in the 00th base according to the data base of NCBI. |
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Revision as of 13:38, 19 September 2012
phaA (beta-ketothiolase orgin from Ralstonia eutropha)
This gene codes the enzyme“Beta- ketothiolase (acetyl-CoA acetyltransferase)”which catalyzes the conversion of two acetyl-CoA molecules into acetoacetyl-CoA. It is an enzyme that catalyzes the first step of P3(HB) producing pathway like the picture below.
This gene was cloned from pGEM phbCAB which carries the synthetic operon genes (phaA,phaB,phaC) of Ralstonia eutropha. In the pGEM phbCAB phaA, we found a mutation in the 00th base according to the data base of NCBI.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 121
Illegal NgoMIV site found at 217
Illegal NgoMIV site found at 225
Illegal NgoMIV site found at 237
Illegal NgoMIV site found at 334
Illegal NgoMIV site found at 574
Illegal NgoMIV site found at 715 - 1000COMPATIBLE WITH RFC[1000]