Difference between revisions of "Part:BBa K759004"
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K759004 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K759004 parameters</partinfo>
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Revision as of 13:30, 19 September 2012

phaA (beta-ketothiolase orgin from Ralstonia eutropha)

This gene codes the enzyme“Beta- ketothiolase (acetyl-CoA acetyltransferase)”which catalyzes the conversion of two acetyl-CoA molecules into acetoacetyl-CoA. It is an enzyme that catalyzes the first step of P3(HB) producing pathway like the picture below.

This gene was cloned from pGEM phbCAB. The pGEM phbCAB carries the synthetic operon genes (phaA,phaB,phaC) of Ralstonia eutropha. In the pGEM phbCAB phaA, we found a mutation in the 00th base according to the data base of NCBI.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 121
    Illegal NgoMIV site found at 217
    Illegal NgoMIV site found at 225
    Illegal NgoMIV site found at 237
    Illegal NgoMIV site found at 334
    Illegal NgoMIV site found at 574
    Illegal NgoMIV site found at 715
  • 1000
    COMPATIBLE WITH RFC[1000]