Difference between revisions of "Part:BBa K777121:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K777121 short</partinfo> | <partinfo>BBa_K777121 short</partinfo> | ||
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===Source=== | ===Source=== | ||
| − | The part was amplified from genomic DNA of E. coli str. K-12 substr. DH10B, complete genome (CP000948.1). | + | * The part was amplified from genomic DNA of E. coli str. K-12 substr. DH10B, complete genome (CP000948.1). |
===References=== | ===References=== | ||
| + | * Paul K., Nieto V., Carlquist W.C., Blair D.F., Harshey R.M. The c-di-GMP Binding Protein YcgR Controls Flagellar Motor Direction and Speed to Affect Chemotaxis by a “Backstop Brake” Mechanism. Mol Cell. 2010. 38: 128–139. | ||
Revision as of 14:46, 18 September 2012
yhjH
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
- yhjH was amplified from genomic DNA of E. coli DH10B via PCR using the following primers:
- Fwd: 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGATAAGGCAGGTTATCCAGC-3'
- Rev: 5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATAGCGCCAGAACCGCCGTATTCAGC-3'
Source
- The part was amplified from genomic DNA of E. coli str. K-12 substr. DH10B, complete genome (CP000948.1).
References
- Paul K., Nieto V., Carlquist W.C., Blair D.F., Harshey R.M. The c-di-GMP Binding Protein YcgR Controls Flagellar Motor Direction and Speed to Affect Chemotaxis by a “Backstop Brake” Mechanism. Mol Cell. 2010. 38: 128–139.