Difference between revisions of "Part:BBa K750000"
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== '''Performance''' ==
 
== '''Performance''' ==
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After completing the construction of this device, we did fluorescence test and protein electrophoresis of gfp(lva). Here is the electrophoresis image of gfp(lva).
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[[Image:Gfp(lva)1.jpg]]
 
== '''Reference''' ==
 
== '''Reference''' ==
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 13:54, 18 September 2012

pBADGLT:unstable gfp expression device activated by arabinose

Description

This part contains Pbad promoter, RBS of 1.0 strength, GFP(lva) producer and double terminator. GFP(lva) will be produced after arabinose added. In the cell display part of our project, this part is used in part...part...part...

Performance

After completing the construction of this device, we did fluorescence test and protein electrophoresis of gfp(lva). Here is the electrophoresis image of gfp(lva). Gfp(lva)1.jpg

Reference

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 800