Difference between revisions of "Part:BBa K731500:Design"

(→‎Design Notes)
(→‎Design Notes)
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This part is composed of K731300 + a ''tac'' promoter followed by a
 
This part is composed of K731300 + a ''tac'' promoter followed by a
 
''lac'' operator. ''lac'' operator sequence corresponds to plasmid pET21b one.
 
''lac'' operator. ''lac'' operator sequence corresponds to plasmid pET21b one.
 +
 +
 
''tac'' promoter and ''lac'' operator were amplified by PCR starting from a synthesized sequence composed of prefix-lacI-lacIq-Ptac-lacO-CysDes-suffix in pUC57.
 
''tac'' promoter and ''lac'' operator were amplified by PCR starting from a synthesized sequence composed of prefix-lacI-lacIq-Ptac-lacO-CysDes-suffix in pUC57.
  

Revision as of 10:22, 17 September 2012

[LacI + LacIq promoter] reverse + [tac promoter + lac operator] forward


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is composed of K731300 + a tac promoter followed by a lac operator. lac operator sequence corresponds to plasmid pET21b one.


tac promoter and lac operator were amplified by PCR starting from a synthesized sequence composed of prefix-lacI-lacIq-Ptac-lacO-CysDes-suffix in pUC57.

Source

K731300 part + Ptac & lacO from pET21b

References