Difference between revisions of "Part:BBa K784029"

 
 
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__NOTOC__
 
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<partinfo>BBa_K784029 short</partinfo>
 
<partinfo>BBa_K784029 short</partinfo>
  
 
This part consists of T3 RNA polymerase induced promoter followed by an RBS and the &#8206;Alkaline Phosphatase reporter gene (pHOA) from Citrobacter.&#8206;
 
This part consists of T3 RNA polymerase induced promoter followed by an RBS and the &#8206;Alkaline Phosphatase reporter gene (pHOA) from Citrobacter.&#8206;
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The part's purpose is to monitor the translation of the T3 RNA polymerase with the Alkaline &#8206;Phosphatase gene which can be quantified with the PNP (para-nitrophenol phosphate) hydrolysis assay that has been &#8206;made by Arkin Lab group in 2006.&#8206;
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The part's purpose is to monitor the translation of the T3 RNA polymerase with the Alkaline &#8206;Phosphatase gene which can be quantified with the [https://parts.igem.org/Arkin_JCA_PNPAssay  PNP (para-nitrophenol phosphate) hydrolysis assay] that has been &#8206;made by Arkin Lab group in 2006.&#8206;
 
The Alkaline Phosphatase + RBS part is between XmaI and XhoI restriction sites and &#8206;therefore can be excised out by these enzymes.
 
The Alkaline Phosphatase + RBS part is between XmaI and XhoI restriction sites and &#8206;therefore can be excised out by these enzymes.
  

Latest revision as of 10:17, 17 September 2012

pT3+RBS+Alkaline Phosphatase (pHO)+Terminator

This part consists of T3 RNA polymerase induced promoter followed by an RBS and the ‎Alkaline Phosphatase reporter gene (pHOA) from Citrobacter.‎ The part's purpose is to monitor the translation of the T3 RNA polymerase with the Alkaline ‎Phosphatase gene which can be quantified with the PNP (para-nitrophenol phosphate) hydrolysis assay that has been ‎made by Arkin Lab group in 2006.‎ The Alkaline Phosphatase + RBS part is between XmaI and XhoI restriction sites and ‎therefore can be excised out by these enzymes.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1512
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1512
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1512
    Illegal XhoI site found at 24
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1512
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1512
    Illegal NgoMIV site found at 351
    Illegal NgoMIV site found at 798
  • 1000
    COMPATIBLE WITH RFC[1000]