Difference between revisions of "Part:BBa B0010:Experience"

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This review comes from the old result system and indicates that this part did not work in some test.  
 
This review comes from the old result system and indicates that this part did not work in some test.  
 
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<partinfo>BBa_B0010 AddReview 0</partinfo>
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<I>Trento iGEM team 2012</I>
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This part was successfully extracted from the distribution kits 2012 and 2011. However, it was not confirmed by restriction digestion nor PCR.  We were able to amplify it by PCR from BBa_E0840, that contains the double terminator BBa_B0015, subcloned it into pSB1C3 and confirmed by sequencing. We re-deposited rrnBT1 terminator well characterized and functioning as [[Part:BBa_K731722|BBa_K731722]].
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Revision as of 15:26, 16 September 2012

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_B0010

  • PCR Problems: Using primers VR and VF2 to PCR B0010 results in excess bands. VR can anneal to B0010, resulting in shorter bands than expected. A full description of the problem is available here.

User Reviews

UNIQd776c756e43e1a7c-partinfo-00000000-QINU

Antiquity

This review comes from the old result system and indicates that this part did not work in some test.


Trento iGEM team 2012

This part was successfully extracted from the distribution kits 2012 and 2011. However, it was not confirmed by restriction digestion nor PCR. We were able to amplify it by PCR from BBa_E0840, that contains the double terminator BBa_B0015, subcloned it into pSB1C3 and confirmed by sequencing. We re-deposited rrnBT1 terminator well characterized and functioning as BBa_K731722.

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UNIQd776c756e43e1a7c-partinfo-00000003-QINU