Difference between revisions of "Part:BBa K861110:Experience"

 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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===Applications of BBa_K861110===
 
===Applications of BBa_K861110===
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As for the genes we clone, there is no difference between <i>E. coli str. K12 MG1655</i> and more available DH5&alpha;. we purified and amplified these genes from genome of <i>Escherichia coli str. DH5&alpha;</i> using PCR. The primers contain the standard restriction enzyme cutting sites. The sequences of the primers used are as below.
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<li>Antisense 5'CCTGCAGTACTAGTATTACTCGTTATCCGGGTTAAGAC3;
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<br/><li>Sense　　5'CGAATTCTTCTAGAGATGAAAAGAAAACTATTCTGGATTTG3'
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<br/>After amplifying by PCR, the DNA fragmentation was examined by agarose gel electrophoresis, the lads show that the sizes of the gene were correct.
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<img src="https://static.igem.org/mediawiki/2012/4/42/BcsB.png" width="100" height="400" hspace="2" vspace="1" border="2" align="CENTER"/>
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===User Reviews===
 
===User Reviews===

Latest revision as of 07:54, 16 September 2012

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K861110

As for the genes we clone, there is no difference between E. coli str. K12 MG1655 and more available DH5α. we purified and amplified these genes from genome of Escherichia coli str. DH5α using PCR. The primers contain the standard restriction enzyme cutting sites. The sequences of the primers used are as below.

  • Antisense 5'CCTGCAGTACTAGTATTACTCGTTATCCGGGTTAAGAC3;
  • Sense　　5'CGAATTCTTCTAGAGATGAAAAGAAAACTATTCTGGATTTG3'
    After amplifying by PCR, the DNA fragmentation was examined by agarose gel electrophoresis, the lads show that the sizes of the gene were correct.

    User Reviews

    UNIQaaba9a2cfda77f53-partinfo-00000001-QINU UNIQaaba9a2cfda77f53-partinfo-00000002-QINU