Difference between revisions of "Part:BBa K861170"
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<partinfo>BBa_K861170 short</partinfo> | <partinfo>BBa_K861170 short</partinfo> | ||
| − | This is a promoter designed for the Eschaerichia coli . It includes the CRP-binding site and the RNA polymerase-binding site which have a overlap of several base pairs. Due to the steric hindrance between CRP and RNA polymerase, gene downstream of the promoter will be repressed.In the cells ,low glucose concentration will result in increased activity by adenylate cyclase, rising up the concentration of cAMP in cell. cAMP binds to the CRP, which, in its bound form, is able to bind tightly to the specific DNA site in the promoter and repress the gene downstream.On the contrary, high glucose concentration will result in the expression of downstream genes. Compared to PII (BBa_K861171), the binding of CRP and the promoter is weaker and the promoter is stronger due to the sequence change of CRP binding site to meet the -10 region of the promoter. | + | This is a promoter designed for the Eschaerichia coli . It includes the CRP-binding site and the RNA polymerase-binding site which have a overlap of several base pairs. Due to the steric hindrance between CRP and RNA polymerase, gene downstream of the promoter will be repressed.In the cells ,low glucose concentration will result in increased activity by adenylate cyclase, rising up the concentration of cAMP in cell. cAMP binds to the CRP, which, in its bound form, is able to bind tightly to the specific DNA site in the promoter and repress the gene downstream. On the contrary, high glucose concentration will result in the expression of downstream genes. Compared to PII (BBa_K861171), the binding of CRP and the promoter is weaker and the promoter is stronger due to the sequence change of CRP binding site to meet the -10 region of the promoter. |
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Revision as of 06:28, 16 September 2012
Status: 500 Content-type: text/html
Software error:
Not enough arguments for Range::range at /websites/parts.igem.org/cgi/lib/Range.pm line 250, near "range;" Unmatched right curly bracket at /websites/parts.igem.org/cgi/lib/Range.pm line 251, at end of line syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 251, near "}" Can't use global @_ in "my" at /websites/parts.igem.org/cgi/lib/Range.pm line 261, near "= @_" syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 282, near "}" Can't use global @_ in "my" at /websites/parts.igem.org/cgi/lib/Range.pm line 286, near "= @_" Global symbol "$course" requires explicit package name at /websites/parts.igem.org/cgi/lib/Range.pm line 288. syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 314, near "}" Compilation failed in require at /websites/parts.igem.org/cgi/lib/Part.pm line 16. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Part.pm line 16. Compilation failed in require at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.
For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.
This is a promoter designed for the Eschaerichia coli . It includes the CRP-binding site and the RNA polymerase-binding site which have a overlap of several base pairs. Due to the steric hindrance between CRP and RNA polymerase, gene downstream of the promoter will be repressed.In the cells ,low glucose concentration will result in increased activity by adenylate cyclase, rising up the concentration of cAMP in cell. cAMP binds to the CRP, which, in its bound form, is able to bind tightly to the specific DNA site in the promoter and repress the gene downstream. On the contrary, high glucose concentration will result in the expression of downstream genes. Compared to PII (BBa_K861171), the binding of CRP and the promoter is weaker and the promoter is stronger due to the sequence change of CRP binding site to meet the -10 region of the promoter.
Sequence and Features Status: 500 Content-type: text/html
Software error:
Not enough arguments for Range::range at /websites/parts.igem.org/cgi/lib/Range.pm line 250, near "range;" Unmatched right curly bracket at /websites/parts.igem.org/cgi/lib/Range.pm line 251, at end of line syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 251, near "}" Can't use global @_ in "my" at /websites/parts.igem.org/cgi/lib/Range.pm line 261, near "= @_" syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 282, near "}" Can't use global @_ in "my" at /websites/parts.igem.org/cgi/lib/Range.pm line 286, near "= @_" Global symbol "$course" requires explicit package name at /websites/parts.igem.org/cgi/lib/Range.pm line 288. syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 314, near "}" Compilation failed in require at /websites/parts.igem.org/cgi/lib/Part.pm line 16. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Part.pm line 16. Compilation failed in require at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.
For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.