Difference between revisions of "Part:BBa K861100:Experience"
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===Applications of BBa_K861100=== | ===Applications of BBa_K861100=== | ||
| − | As for the genes we clone, there is no difference between E. coli str. K12 MG1655 and more available DH5α we purified and amplified these genes from genome of Escherichia coli str. DH5α using PCR. The primers contain the standard restriction enzyme cutting sites. The sequences of the primers used are as below. | + | As for the genes we clone, there is no difference between E. coli str. K12 MG1655 and more available DH5α. we purified and amplified these genes from genome of Escherichia coli str. DH5α using PCR. The primers contain the standard restriction enzyme cutting sites. The sequences of the primers used are as below. |
<li>Antisense CCTGCAGTACTAGTATCATTGTTGAGCCAAAGCCTG | <li>Antisense CCTGCAGTACTAGTATCATTGTTGAGCCAAAGCCTG | ||
<br/><li>Sense CGAATTCTTCTAGAGATGAGTATCCTGACCCGGTGG | <br/><li>Sense CGAATTCTTCTAGAGATGAGTATCCTGACCCGGTGG | ||
| + | After amplifying by PCR, the DNA fragmentation was examined by agarose gel electrophoresis, the lads show that the sizes of the gene were correct. | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 11:51, 15 September 2012
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Applications of BBa_K861100
As for the genes we clone, there is no difference between E. coli str. K12 MG1655 and more available DH5α. we purified and amplified these genes from genome of Escherichia coli str. DH5α using PCR. The primers contain the standard restriction enzyme cutting sites. The sequences of the primers used are as below.
User Reviews
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