Difference between revisions of "Part:BBa K731722:Design"
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<partinfo>BBa_K731722 short</partinfo> | <partinfo>BBa_K731722 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
− | + | The terminator is in the standard backbone [[Part:pSB1C3|pSB1C3]]. | |
− | + | Note: it is useful to use TBE1X instead of TAE1X to make screening gels of such small fragments. | |
− | + | ||
===Source=== | ===Source=== | ||
− | This part comes from | + | This part comes from [[Part:BBa_E0840|BBa_E0840]] that contains [[Part:BBa_B0015|BBa_B0015]], a double terminator in which [[Part:BBa_B0010|BBa_B0010]] is the second one. An extraction PCR has been used to isolate this terminator, that then has been inserted in [[Part:pSB1C3|pSB1C3]]. |
===References=== | ===References=== |
Latest revision as of 10:19, 15 September 2012
T1 terminator from E. coli rrnB
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The terminator is in the standard backbone pSB1C3. Note: it is useful to use TBE1X instead of TAE1X to make screening gels of such small fragments.
Source
This part comes from BBa_E0840 that contains BBa_B0015, a double terminator in which BBa_B0010 is the second one. An extraction PCR has been used to isolate this terminator, that then has been inserted in pSB1C3.