Difference between revisions of "Part:BBa K731722:Design"

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===Source===
 
===Source===
  
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This part comes from E0840 that contains B0012, a double terminator in which B0010 is the second one. We used an extraction PCR.
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This part comes from [[Part:BBa_E0840|BBa_E0840]] that contains [[Part:BBa_B0015|BBa_B0015]], a double terminator in which [[Part:BBa_B0010|BBa_B0010]] is the second one. An extraction PCR has been used to isolate this terminator, that then has been inserted in [[Part:pSB1C3|pSB1C3]].
  
 
===References===
 
===References===

Revision as of 10:18, 15 September 2012

T1 terminator from E. coli rrnB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The terminator is in the standard backbone pSB1C3. Note: it is useful to use TBE1X instead of TAE1X to make screening gels of such small fragments.

Source

This part comes from BBa_E0840 that contains BBa_B0015, a double terminator in which BBa_B0010 is the second one. An extraction PCR has been used to isolate this terminator, that then has been inserted in pSB1C3.

References