Difference between revisions of "Part:BBa K818000:Design"

 
(→‎Design Notes)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K818000 short</partinfo>
 
<partinfo>BBa_K818000 short</partinfo>
Line 7: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
Biobrick ready
+
This part backbone plasmid was designed from psac-cm ''Bacillus subtilis'' backbone plasmid. It has
−
 
+
−
 
+
  
 
===Source===
 
===Source===

Revision as of 12:55, 14 September 2012

Integration vector for Bacillus subtilis derived from pSac-Cm


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 5207
    Illegal XbaI site found at 5218
    Illegal SpeI site found at 1
    Illegal PstI site found at 12
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 5207
    Illegal SpeI site found at 1
    Illegal PstI site found at 12
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 5207
    Illegal BamHI site found at 180
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 5207
    Illegal XbaI site found at 5218
    Illegal SpeI site found at 1
    Illegal PstI site found at 12
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 5207
    Illegal XbaI site found at 5218
    Illegal SpeI site found at 1
    Illegal PstI site found at 12
    Illegal NgoMIV site found at 4124
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 2051
    Illegal SapI.rc site found at 3133


Design Notes

This part backbone plasmid was designed from psac-cm Bacillus subtilis backbone plasmid. It has

Source

MolGen Group (University of Groningen)

References