Difference between revisions of "Part:BBa K784001"

 
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<partinfo>BBa_K784001 short</partinfo>
 
<partinfo>BBa_K784001 short</partinfo>

Revision as of 14:27, 13 September 2012

pT7_RBS_xyIE reporter gene

This part's purpose is to testify to the presence of the T7 RNA Polymerase by the xyIE reporter gene from iGEM2006_Edinburgh. It consists of an RNA T7 promoter, an RBS, a reporter gene- xyIE, and a T7 terminator. The assay for xyIE is very simple, refer to the xyIE basic part for instructions. The xyIE+RBS part is between XhoI and XmaI restriction sites, therefore can be excised by these enzymes.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 368
    Illegal NgoMIV site found at 540
    Illegal AgeI site found at 891
  • 1000
    COMPATIBLE WITH RFC[1000]