Difference between revisions of "Part:BBa K801999:Design"
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===References=== | ===References=== | ||
− | <!-- Here you find templates to insert references to important publications, | + | <!-- Here you find templates to insert references to important publications, GenBank entries, PDB structures and so on--> |
'''Literature references:'''<br> | '''Literature references:'''<br> | ||
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'''Sequence references:'''<br> | '''Sequence references:'''<br> | ||
− | <!--*[http://www.ncbi.nlm.nih.gov/nuccore/?accessNr? ''' | + | <!--*[http://www.ncbi.nlm.nih.gov/nuccore/?accessNr? '''GenBank''': ?title?]--> |
<!--*[http://www.ebi.ac.uk/interpro/IEntry?ac=?accessNr? '''Interpro''': ?title?]--> | <!--*[http://www.ebi.ac.uk/interpro/IEntry?ac=?accessNr? '''Interpro''': ?title?]--> | ||
<!--*[http://www.uniprot.org/uniprot/?accessNr? '''Uniprot''': ?title?]--> | <!--*[http://www.uniprot.org/uniprot/?accessNr? '''Uniprot''': ?title?]--> | ||
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<br>'''Keywords:''' | <br>'''Keywords:''' | ||
− | fluorescent, reporter, chromophore, luminescence, bioluminescence, photoprotein | + | fluorescent, reporter, chromophore, luminescence, bioluminescence, photoprotein |
<br>'''Used abbreviations:''' | <br>'''Used abbreviations:''' | ||
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===References=== | ===References=== | ||
− | <!-- Here you find templates to insert references to important publications, | + | <!-- Here you find templates to insert references to important publications, GenBank entries, PDB structures and so on--> |
'''Literature references:'''<br> | '''Literature references:'''<br> | ||
*[http://www.ncbi.nlm.nih.gov/pubmed/20010584 '''Pubmed:''' Prasher, 1995: Using GFP to see the light.(Review)] | *[http://www.ncbi.nlm.nih.gov/pubmed/20010584 '''Pubmed:''' Prasher, 1995: Using GFP to see the light.(Review)] | ||
− | *[http://www.ncbi.nlm.nih.gov/pubmed/7854443 '''Pubmed''': Heim, 1995: Improved green fluorescence.(Reference for | + | *[http://www.ncbi.nlm.nih.gov/pubmed/7854443 '''Pubmed''': Heim, 1995: Improved green fluorescence.(Reference for Chromophore)] |
'''Sequence references:'''<br> | '''Sequence references:'''<br> | ||
− | *[http://www.ncbi.nlm.nih.gov/nuccore/X83959.1 ''' | + | *[http://www.ncbi.nlm.nih.gov/nuccore/X83959.1 '''GenBank''': A.victoria mRNA for green fluorescent protein (ID:gfp1)] |
*[http://www.ebi.ac.uk/interpro/IEntry?ac=IPR011584 '''Interpro''': Green fluorescent protein-related ] | *[http://www.ebi.ac.uk/interpro/IEntry?ac=IPR011584 '''Interpro''': Green fluorescent protein-related ] | ||
*[http://www.uniprot.org/uniprot/P42212 '''Uniprot''': Green fluorescent protein] | *[http://www.uniprot.org/uniprot/P42212 '''Uniprot''': Green fluorescent protein] |
Revision as of 12:50, 13 September 2012
Test page for standardized BioBrick part descriptions
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Keywords:
Abbreviations:
Design Notes
Other versions of this BioBrick:
Cloning details:
Protein coding:
Enzymatic activity:
Cytotoxicity:
Source
Source:
Organism:
References
Literature references:
Sequence references:
Structure reference:
Test page for standardized BioBrick part descriptions
Keywords:
fluorescent, reporter, chromophore, luminescence, bioluminescence, photoprotein
Used abbreviations:
- GFP = Green Fluorescent Protein
Design Notes
Other versions of this BioBrick:
- The BioBrick BBa_I757008 encodes a yellow fluorescent protein (mVenus) derived from GFP
- The BioBrick BBa_I757008 encodes GFP in RFC25 for protein fusions
Cloning details:
- Part was designed in RFC10
- Mutation G381A to delete XbaI restriction site
- The correctness of the part was checked by sequencing
Protein coding:
- Green Fluorescent Protein [Nucleotide 1 to 714]
- The protein has the amino acid replacements Ser65Thr in order to increase fluorescence, photostability and to shift the major excitation peak to 488 nm. (see Heim et al., 1995)
- The protein encoded is posttranslationally modified by a cross-link between Ser65 and Gly67 to form the chromophore.
Enzymatic activity: none
Cytotoxicity: none
Source
Source:
- Amplified from plasmid: pSB1C3-GFP-generator, provided by Osamu Shimomura, Boston University School of Medicine, USA
Forward Primer:
5'- ATGATGATGATG - 3'
Reverse Primer:
5'- ATGATGATGATG - 3'
Organism:
- Sequence derived from Aequorea victoria
- Codon optimized for Escherichia coli
References
Literature references:
- [http://www.ncbi.nlm.nih.gov/pubmed/20010584 Pubmed: Prasher, 1995: Using GFP to see the light.(Review)]
- [http://www.ncbi.nlm.nih.gov/pubmed/7854443 Pubmed: Heim, 1995: Improved green fluorescence.(Reference for Chromophore)]
Sequence references:
- [http://www.ncbi.nlm.nih.gov/nuccore/X83959.1 GenBank: A.victoria mRNA for green fluorescent protein (ID:gfp1)]
- [http://www.ebi.ac.uk/interpro/IEntry?ac=IPR011584 Interpro: Green fluorescent protein-related ]
- [http://www.uniprot.org/uniprot/P42212 Uniprot: Green fluorescent protein]
- [http://pfam.sanger.ac.uk/family/PF01353 Pfam: Green fluorescent protein]
Structure reference:
- [http://www.rcsb.org/pdb/explore/explore.do?structureId=1EMA PDB: Green Fluorescent Protein from Aequorea victoria]