Difference between revisions of "Part:BBa K777000:Design"

Revision as of 20:51, 11 September 2012

Tar receptor

Assembly Compatibility:

@@ Line 6: / Line 6: @@
 ===Design Notes===
-Genomic sequence was amplified using the following primers.<br><br>
-Primers PCR: Base pairs in caps represent the respective restriction sites followed by either prefix or suffix. Primers were provided by SIGMA.
-Fwd: Tar for + prefix: 5´ actGAATTCgcggccgctTCTAGAtgattaaccgtatccgcgtagtc 3´
-Rev: Tar rev + suffix: 5´ tcCTGCAGcggccgctACTAGTcaaaatgtttcccagtttggatc 3´
-<br><br>
-<br><br>
-One XbaI site was found at position 420. The restriction site was changed from TCTAGt to CCTAGt via QuikChange PCR. <br><br>
-Primers QuikChange: Primers were provided by SIGMA.
-Fwd: TarQC for: 5´ ACTGATTGATTACCTAGATTATGGCAATACTGGAG 3´
-Rev: TarQC rev: 5´ TGCCATAATCTAGGTAATCAATCAGTTCAGTTAAC 3´
 ===Source===
-The part was amplified from genomic DNA of <i>E. coli</i> str. K-12 substr. DH10B chromosome (NC_010473.1).
 ===References===