Difference between revisions of "Part:BBa K801999:Design"
VolkerMorath (Talk | contribs) (→Source) |
VolkerMorath (Talk | contribs) |
||
Line 62: | Line 62: | ||
<br>'''Keywords:''' | <br>'''Keywords:''' | ||
− | fluorescent, reporter | + | fluorescent, reporter, chromophore, luminescence, bioluminescence, photoprotein |
+ | |||
<br>'''Abbreviations:''' | <br>'''Abbreviations:''' | ||
*GFP = Green Fluorescent Protein | *GFP = Green Fluorescent Protein | ||
===Design Notes=== | ===Design Notes=== | ||
+ | |||
'''Other versions of this BioBrick:''' | '''Other versions of this BioBrick:''' | ||
*The BioBrick BBa_I757008 encodes a yellow fluorescent protein (mVenus)derived from GFP. | *The BioBrick BBa_I757008 encodes a yellow fluorescent protein (mVenus)derived from GFP. | ||
Line 90: | Line 92: | ||
'''Source:''' | '''Source:''' | ||
*Amplified from plasmid: pSB1C3-GFP-generator, provided by A. Skerra, TU Munich, Germany | *Amplified from plasmid: pSB1C3-GFP-generator, provided by A. Skerra, TU Munich, Germany | ||
+ | |||
'''Forward Primer:'''<br> | '''Forward Primer:'''<br> | ||
<code>5'- ??? - 3'</code><br> | <code>5'- ??? - 3'</code><br> |
Revision as of 20:08, 11 September 2012
Test page for standardized BioBrick part descriptions
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Keywords:
Abbreviations:
Design Notes
Other versions of this BioBrick:
Cloning details:
Protein coding:
Enzymatic activity:
Cytotoxicity:
Source
Source:
Organism:
References
Literature references:
Seqeuence references:
Structure reference:
Test page for standardized BioBrick part descriptions
Keywords:
fluorescent, reporter, chromophore, luminescence, bioluminescence, photoprotein
Abbreviations:
- GFP = Green Fluorescent Protein
Design Notes
Other versions of this BioBrick:
- The BioBrick BBa_I757008 encodes a yellow fluorescent protein (mVenus)derived from GFP.
- The BioBrick BBa_I757008 encodes GFP in RFC25 for protein fusions.
Cloning details:
- Part was designed in RFC10.
- Mutation G381A to delete XbaI restriction site
- The correctness of part was checked by sequencing.
Protein coding:
- Green Fluorescent Prtein [Nucleotide 1 to 714]>
- The protein has the amino acid replacements Ser65Thr in order to increased fluorescence, photostability, and to shift the major excitation peak to 488 nm. (see Heim et all., 1995)
- The protein encoded is posttranslationally modified by a cross-link between Ser65 and Gly67 to form the chromophor.
Enzymatic activity: none
Cytotoxicity: none
Source
Source:
- Amplified from plasmid: pSB1C3-GFP-generator, provided by A. Skerra, TU Munich, Germany
Forward Primer:
5'- ??? - 3'
Reverse Primer:
5'- ??? - 3'
Organism:
- Genesequence derived from Aequorea victoria
- Codonoptimized for Escherichia coli
References
Literature references:
- [http://www.ncbi.nlm.nih.gov/pubmed/20010584 Pubmed: Prasher, 1995: Using GFP to see the light.(Review)]
- [http://www.ncbi.nlm.nih.gov/pubmed/7854443: Heim, 1995: Improved green fluorescence.(Reference for Chromophor)]
Seqeuence references:
- [http://www.ncbi.nlm.nih.gov/nuccore/X83959.1 GeneBank: A.victoria mRNA for green fluorescent protein (ID:gfp1)]
- [http://www.ebi.ac.uk/interpro/IEntry?ac=IPR011584 Interpro: Green fluorescent protein-related ]
- [http://www.uniprot.org/uniprot/P42212 Uniprot: Green fluorescent protein]
- [http://pfam.sanger.ac.uk/family/PF01353 Pfam: Green fluorescent protein]
Structure reference:
- [http://www.rcsb.org/pdb/explore/explore.do?structureId=1EMA PDB: Green Fluorescent Protein from Aequorea victoria]