Difference between revisions of "Part:BBa K861061"

 
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<partinfo>BBa_K861061 short</partinfo>
 
<partinfo>BBa_K861061 short</partinfo>
  
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Promoter + BBa_I13507 in high copy number plasmid. Used to amplify plasmid
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We use BBa_I13507 test function of BBa_K861060 (PfadR) in M9 medium with oleic acid as sole carbon source in plasmid pSB6A1. Specifically, oleic acid was emulsified with 10% Triton X100 with volume 1:1. Then various volume of mixture is added to M9 medium with 0.2% triton X100. We vortex E.coli Dh5 alpha in the medium for 24h and using SpectraMax M2 plate reader to see its OD600 and fluorescence.
  
 
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Revision as of 05:17, 11 September 2012

Efficacy testing RFP generator of BBa_K861060 (PfadR)

We use BBa_I13507 test function of BBa_K861060 (PfadR) in M9 medium with oleic acid as sole carbon source in plasmid pSB6A1. Specifically, oleic acid was emulsified with 10% Triton X100 with volume 1:1. Then various volume of mixture is added to M9 medium with 0.2% triton X100. We vortex E.coli Dh5 alpha in the medium for 24h and using SpectraMax M2 plate reader to see its OD600 and fluorescence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 657
    Illegal AgeI site found at 769
  • 1000
    COMPATIBLE WITH RFC[1000]