Difference between revisions of "Part:BBa K911001:Design"

 
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===References===
 
===References===
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*Dann C., Wakeman C., Sieling C. Baker S. Irnov I. and Winkler W., Structure and Mechanism of a Metal-Sensing Regulatory RNA, Cell ''130'', 878–892

Latest revision as of 14:34, 7 September 2012

Magnesium sensitive riboswitch


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 452
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Original paper mentioned 8 codon substitution of the downstream LacZ reporter gene with the first 8 codons of the MgtE gene (which is the native gene which this riboswitch controls). Our construct used LacI as the downstream regulated sequence, which requires its N-terminal domain to bind to DNA. Sequences both with and without this substitution have been submitted. This copy does not contain the substitution.


Source

Bacillus Subtilis Genome

References

  • Dann C., Wakeman C., Sieling C. Baker S. Irnov I. and Winkler W., Structure and Mechanism of a Metal-Sensing Regulatory RNA, Cell 130, 878–892