Difference between revisions of "Part:BBa K268000:Experience"

Revision as of 23:38, 6 September 2012

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Applications of BBa_K268000

User Reviews

Earthnut

WARNING: THIS PART STILL CONTAINS AT LEAST ONE FORBIDDEN RESTRICTION SITE (XbaI or PstI) IN THE BACKBONE! ALSO THE PREDICTION ALGORITHM FOR FORBIDDEN SITES DO NOT WORK PROPERLY IN THIS CASE. AT BASEPAIR 46 there is a NotI RESTRICTION SITE OF RFC10.

An analytical digestion with XbaI and PstI was performed to linearize the plasmid, but unexpectedly there was more than one band visible, one at ~3.4 kbp and one at ~1.4 kbp (3.4 kbp + 1.4 kbp = 6.8 kbp).

TU_MUNICH 2012

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UNIQ98e3b41b8d82c72f-partinfo-00000001-QINU UNIQ98e3b41b8d82c72f-partinfo-00000002-QINU

@@ Line 15: / Line 15: @@
 <span style="color: red"> WARNING: </span>THIS PART STILL CONTAINS AT LEAST ONE FORBIDDEN RESTRICTION SITE (XbaI or PstI) IN THE BACKBONE! ALSO THE PREDICTION ALGORITHM FOR FORBIDDEN SITES DO NOT WORK PROPERLY IN THIS CASE. AT BASEPAIR 46 there is a NotI RESTRICTION SITE OF RFC10.
-An analytical digestion with XbaI and PstI was performed to linearize the plasmid, but unexpectedly there was more than one band visible, one at ~3.4&nbsp;kbp and one at ~1.4&nbsp;kbp.
+An analytical digestion with XbaI and PstI was performed to linearize the plasmid, but unexpectedly there was more than one band visible, one at ~3.4&nbsp;kbp and one at ~1.4&nbsp;kbp (3.4&nbsp;kbp&nbsp;+&nbsp;1.4&nbsp;kbp&nbsp;=&nbsp;6.8&nbsp;kbp).
 [[Image:TUM12 20120906 anal gel P674-677x.jpg|300px]]