Difference between revisions of "Part:BBa K861030"

(New page: __NOTOC__ <partinfo>BBa_K861020 short</partinfo> The first step in the beta-oxidation pathway involves the conversion of acyl-CoA to enoyl-CoA .This process is catalyzed by acyl-CoA dehyd...)
 
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The first step in the beta-oxidation pathway involves the conversion of acyl-CoA to enoyl-CoA .This process is catalyzed by acyl-CoA dehydrogenase (FadE). Its action results in the introduction of a trans double-bond between C2 and C3 of the acyl-CoA thioester substrate. And it is repressed by FadR.
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For a E.coli to degrade fatty acid, at least for enzymes – FadA, FadB, FadD, FadE and transporter—FadL are needed. Long chain fatty acids are firstly being imported by the transmembrane protein FadL. After FAs get into cells, they will be added a CoA by inner membrane-associated FadD (acyl-CoA synthase). β-oxidation is initiated by FadE(acyl-CoA dehydrogenase), which will convert acyl-CoA into enoyl-CoA. The followed cycles of hydration, oxidation, and thiolytic cleavage are carried out by tetrameric complex consisting of two FadA and two FadB proteins.
 
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===Usage and Biology===
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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===Functional Parameters===
 
===Functional Parameters===
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Revision as of 06:38, 6 September 2012

FadB, gene for fatty acid degradation from E.coli K12

For a E.coli to degrade fatty acid, at least for enzymes – FadA, FadB, FadD, FadE and transporter—FadL are needed. Long chain fatty acids are firstly being imported by the transmembrane protein FadL. After FAs get into cells, they will be added a CoA by inner membrane-associated FadD (acyl-CoA synthase). β-oxidation is initiated by FadE(acyl-CoA dehydrogenase), which will convert acyl-CoA into enoyl-CoA. The followed cycles of hydration, oxidation, and thiolytic cleavage are carried out by tetrameric complex consisting of two FadA and two FadB proteins. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 150
    Illegal BglII site found at 297
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]