Difference between revisions of "Part:BBa K731700"

Revision as of 23:57, 30 August 2012

Platform for terminators analysis under the control of T7 promoter

This part is used to analyse terminator efficiency. It was built starting from pET21b vector containing T7 promoter - mCherry - A 206K Venus. Two illegal sites were removed and the prefix and suffix sites were inserted between the two fluorescent proteins. The platform has been successfully used for the ratiometric analysis of terminator efficiency.

The combined use of BBa_K731710 and BBa_K731700 allow also to analyse any potential difference in terminators' activity due to different polymerases.

Usage and Biology

Protocol

	Excitation (nm)	Emission(nm)
mCherry	587	610
Venus	515	528

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 6850
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 6856
21
INCOMPATIBLE WITH RFC[21]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 6850
Illegal BglII site found at 6011
Illegal BamHI site found at 6832
Illegal XhoI site found at 753
23
INCOMPATIBLE WITH RFC[23]
Illegal prefix found at 6850
Illegal suffix found at 2
25
INCOMPATIBLE WITH RFC[25]
Illegal prefix found at 6850
Plasmid lacks a suffix.
Illegal XbaI site found at 6865
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NgoMIV site found at 714
Illegal NgoMIV site found at 1051
Illegal NgoMIV site found at 4231
Illegal NgoMIV site found at 4391
Illegal NgoMIV site found at 5979
Illegal AgeI site found at 6800
1000
INCOMPATIBLE WITH RFC[1000]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 2228
Illegal SapI.rc site found at 3310

@@ Line 9: / Line 9: @@
 ===Usage and Biology===
+Protocol
 {|border = "1" cellpadding="5" cellspacing="0" align="left"
@@ Line 23: / Line 25: @@
 |528
 |}