Difference between revisions of "Part:BBa K754000:Design"

(→‎Design Notes)
(→‎Design Notes)
 
(One intermediate revision by the same user not shown)
Line 6: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
No special design consideration were taken during the biobrick designing. At any moment was necesarry because apparently this fragment had no tarqet sequences for the standard restriction enzymes used and recomended by the parts registry. Despite of our initial cdonsideration, the biobrick has target sequences for three of the enzymes
+
Certain punctual changes on the promoter sequence had to be done in order tu ensure that the biobrick was compatible with all the registry standarized enzymes. Specifically the third nucelotide of the sequence (initially a guanine) had to be changed by an adenine in order to remove an XbaI target
  
 
===Source===
 
===Source===

Latest revision as of 18:21, 29 August 2012

S. elongatus PCC7942 psbAI promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Certain punctual changes on the promoter sequence had to be done in order tu ensure that the biobrick was compatible with all the registry standarized enzymes. Specifically the third nucelotide of the sequence (initially a guanine) had to be changed by an adenine in order to remove an XbaI target

Source

The sequence of this part comes from the AM 2195 plasmid developed by doctor Susan Golden.

References