Difference between revisions of "Part:BBa K731520"

 
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<partinfo>BBa_K731520 short</partinfo>
 
<partinfo>BBa_K731520 short</partinfo>
  
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This is a construct carrying the sfGFP gene with low-efficiency RBS, under lac operator control.
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This part is composed of K731500 and E0240 and allows the
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characterization of K731500. It was successfully operated and characterized
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lac repressor (encoded from lacI gene) is the regulator protein: in absence of lactose the protein binds to lac operator, preventing RNA-polymerase binding and transcription.Low, constitutive levels of transcription can be seen anyhow.
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in both pSB4K5 and pSB1C3.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 08:14, 29 August 2012

IPTG inducible GFPmut3b

This part is composed of K731500 and E0240 and allows the characterization of K731500. It was successfully operated and characterized in both pSB4K5 and pSB1C3. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1910