Difference between revisions of "Part:BBa K524203:Design"
(No difference)

Revision as of 11:59, 26 August 2012


pSC101 heat sensitive origin of replication with deletion in par locus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1709
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The par locus was deleted through excision of BBa_K524000 by SspI and StuI, purification of desired fragments and then


Source

Modified from BBa_K524000

References