Difference between revisions of "Part:BBa K731710"

Revision as of 07:59, 24 August 2012

Platform for terminators analysis under the control of tac promoter

This part is used to analyse terminators: two subsequent fluorescent proteins (mCherry and A206K Venus) allow standardization of terminator's presence effect. The vector was obained by mutagenesis of Part:BBa_K731700, replacing T7 promoter with pTAC. It contains Ampicillin resistance gene and is inducible by IPTG (lacO). The combined use of BBa_K731710 and Part:BBa_K731700 allow also to analyse any potential difference in terminators' activity due to different polymerases.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 6865
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 6871
21
INCOMPATIBLE WITH RFC[21]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 6865
Illegal BglII site found at 6011
Illegal BamHI site found at 6847
Illegal XhoI site found at 753
23
INCOMPATIBLE WITH RFC[23]
Illegal prefix found at 6865
Illegal suffix found at 2
25
INCOMPATIBLE WITH RFC[25]
Illegal prefix found at 6865
Plasmid lacks a suffix.
Illegal XbaI site found at 6880
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NgoMIV site found at 714
Illegal NgoMIV site found at 1051
Illegal NgoMIV site found at 4231
Illegal NgoMIV site found at 4391
Illegal NgoMIV site found at 5979
Illegal AgeI site found at 6815
1000
INCOMPATIBLE WITH RFC[1000]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 2228
Illegal SapI.rc site found at 3310

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K731710 short</partinfo>
-This part is used to analyse terminators: two subsequent fluorescent proteins (mCherry and A206K Venus)allow to obtain several information about terminator's presence effect.
+This part is used to analyse terminators: two subsequent fluorescent proteins (mCherry and A206K Venus) allow standardization of terminator's presence effect.
 The vector was obained by mutagenesis of [[Part:BBa_K731700]], replacing T7 promoter with pTAC. It contains Ampicillin resistance gene and is inducible by IPTG (lacO).
 The combined use of BBa_K731710 and [[Part:BBa_K731700]] allow also to analyse any potential difference in terminators' activity due to different polymerases.