Difference between revisions of "Part:BBa K731722:Design"

(Design Notes)
(Source)
Line 11: Line 11:
 
===Source===
 
===Source===
  
This part comes from B0012 contained in E0840, a double terminator in which B0010 is the second one. We used an extraction PCR.
+
This part comes from E0840 that contains B0012, a double terminator in which B0010 is the second one. We used an extraction PCR.
  
 
===References===
 
===References===

Revision as of 20:42, 23 August 2012

T1 terminator from E. coli rrnB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The terminator is in the standard backbone pSB1C3. Note: it is useful to use TBE1X instead of TAE1X to make screening gels of such small fragments.

Source

This part comes from E0840 that contains B0012, a double terminator in which B0010 is the second one. We used an extraction PCR.

References