Difference between revisions of "Part:BBa K731030"
Line 3: | Line 3: | ||
The CysE M256I gene ([https://parts.igem.org/Part:BBa_K731010 K731010]) is here regulated by the araC-pBAD promoter ([https://parts.igem.org/Part:BBa_K731200 K731200]), which is inducible by arabinose. | The CysE M256I gene ([https://parts.igem.org/Part:BBa_K731010 K731010]) is here regulated by the araC-pBAD promoter ([https://parts.igem.org/Part:BBa_K731200 K731200]), which is inducible by arabinose. | ||
+ | This part was sub cloned and characterized in psB3C5. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 08:08, 23 August 2012
Inducible araC-pBAD promoter regulating M256I CysE
The CysE M256I gene (K731010) is here regulated by the araC-pBAD promoter (K731200), which is inducible by arabinose. This part was sub cloned and characterized in psB3C5.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
Illegal AgeI site found at 1909 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961