Difference between revisions of "Part:BBa K823024"
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Revision as of 10:14, 21 August 2012
pSBBs4S-Pxyl: Integrative expression vector for Bacillus subtilis
This part is an expression vector for Bacillus subtilis. It integrates at the thrC locus. It has a ampicillin resistance for cloning in E.coli and chloramphenicol resistance for selection in B.subtilis. The multiple cloning site downstream of a Pxyl promoter, that is inducible with xylose. This BioBrick is part of the LMU 2012 igem project Bacillus BioBrick Box http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 4773
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 4779 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 4773
Illegal BamHI site found at 4757
Illegal XhoI site found at 1011
Illegal XhoI site found at 1929 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 4773
Illegal suffix found at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 4773
Plasmid lacks a suffix.
Illegal XbaI site found at 4788
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NgoMIV site found at 1893
Illegal NgoMIV site found at 3032 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI.rc site found at 2856
Illegal SapI.rc site found at 605