Difference between revisions of "Part:BBa K731030:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | K731200 and K731010 were inserted into pSB1C3 using a variation of the 3A Assembly, which added a PCR amplification step of the inserts before digestion to increase efficiency. The former was cut with E&S, the latter with X&P. pSB1C3 was digested with E&P. | + | [https://parts.igem.org/Part:BBa_K731200 K731200] and [https://parts.igem.org/Part:BBa_K731010 K731010] were inserted into pSB1C3 using a variation of the 3A Assembly, which added a PCR amplification step of the inserts before digestion to increase efficiency. The former was cut with E&S, the latter with X&P. pSB1C3 was digested with E&P. |
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===Source=== | ===Source=== | ||
Revision as of 08:30, 21 August 2012
Inducible araC-pBAD promoter regulating M256I CysE
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
Illegal AgeI site found at 1909 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
Design Notes
K731200 and K731010 were inserted into pSB1C3 using a variation of the 3A Assembly, which added a PCR amplification step of the inserts before digestion to increase efficiency. The former was cut with E&S, the latter with X&P. pSB1C3 was digested with E&P.
Source
K731200, K731010