Difference between revisions of "Part:BBa K823020:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | We constructed the part in the freiburg standard (Assembly 25). We included a Bacillus optimized RBS between the XbaI and NgoIV restriction site. | + | We constructed the part in the freiburg standard (Assembly 25). We included a Bacillus optimized RBS between the XbaI and NgoIV restriction site. |
| − | + | The part is delivered in pSB1A3 to avoid the same antibiotic resistance twice on a plasmid. | |
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===Source=== | ===Source=== | ||
Latest revision as of 16:27, 15 August 2012
chloramphenicol acetyltransferase (cat)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We constructed the part in the freiburg standard (Assembly 25). We included a Bacillus optimized RBS between the XbaI and NgoIV restriction site. The part is delivered in pSB1A3 to avoid the same antibiotic resistance twice on a plasmid.
Source
Plasmid: Bacillus subtilis reporter vector pAC6