Difference between revisions of "Part:BBa K734000:Design"
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<partinfo>BBa_K734000 short</partinfo> | <partinfo>BBa_K734000 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
− | + | Genes and were amplified and assembled using Gibson isothermal assembly. BBa_K515105 was used as a source for the J23100 promoter + pSB1C3 backbone. | |
+ | ===Source=== | ||
+ | Methylxanthine N-demethylases ''NdmA'',''NdmB'',''NdmC'' and associated reductase ''NdmD'' were amplified from ''Psuedomonas putida CBB5'' genomic DNA. | ||
− | + | ''gst9'' from ''Janthinobacterium sp. Marseille'' was synthesized. | |
− | + | ||
− | + | ||
===References=== | ===References=== | ||
+ | <biblio> | ||
+ | #Summers2012 pmid=22328667 | ||
+ | #Summers2011 pmid=20966097 | ||
+ | #Yu2009 pmid=19447909 | ||
+ | </biblio> |
Latest revision as of 20:49, 14 August 2012
Caffeine demethylation pathway
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1229
Illegal XbaI site found at 1126
Illegal XbaI site found at 2229
Illegal XbaI site found at 3109
Illegal PstI site found at 548 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1229
Illegal NheI site found at 8
Illegal NheI site found at 31
Illegal NheI site found at 1730
Illegal NheI site found at 1925
Illegal PstI site found at 548 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1229
Illegal BglII site found at 2687
Illegal BglII site found at 3181
Illegal BglII site found at 3652
Illegal XhoI site found at 4793 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1229
Illegal XbaI site found at 1126
Illegal XbaI site found at 2229
Illegal XbaI site found at 3109
Illegal PstI site found at 548 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1229
Illegal XbaI site found at 1126
Illegal XbaI site found at 2229
Illegal XbaI site found at 3109
Illegal PstI site found at 548
Illegal NgoMIV site found at 5491
Illegal NgoMIV site found at 5604
Illegal AgeI site found at 2224
Illegal AgeI site found at 3320 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 984
Illegal BsaI.rc site found at 2221
Design Notes
Genes and were amplified and assembled using Gibson isothermal assembly. BBa_K515105 was used as a source for the J23100 promoter + pSB1C3 backbone.
Source
Methylxanthine N-demethylases NdmA,NdmB,NdmC and associated reductase NdmD were amplified from Psuedomonas putida CBB5 genomic DNA.
gst9 from Janthinobacterium sp. Marseille was synthesized.
References
<biblio>
- Summers2012 pmid=22328667
- Summers2011 pmid=20966097
- Yu2009 pmid=19447909
</biblio>