Difference between revisions of "Part:BBa K731201"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K731200 short</partinfo> | <partinfo>BBa_K731200 short</partinfo> | ||
| − | This E. coli promoter is positively regulated by arabinose. araC is the regulator protein | + | This E. coli promoter is positively regulated by arabinose. araC is the regulator protein: in absence of arabinose the protein binds to araI1 and araO2 and the DNA forms a loop that prevents RNA polymerase from binding the promoter. |
| + | When arabinose is added to the culture it binds to araC, disabling the interaction with araI1 and araO2 sites, and also allowing it to bind araI site, promoting transcription. | ||
| + | Promoter activity is also influenced by glucose levels: in its absence cAMP levels are high and the the complex cAMP+CAP (catabolite activator protein) binds to lacI promoting transcription. In presence of glucose, cAMP levels are low and promoter activity is inhibited. | ||
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Revision as of 10:19, 8 August 2012
No part name specified with partinfo tag.
This E. coli promoter is positively regulated by arabinose. araC is the regulator protein: in absence of arabinose the protein binds to araI1 and araO2 and the DNA forms a loop that prevents RNA polymerase from binding the promoter. When arabinose is added to the culture it binds to araC, disabling the interaction with araI1 and araO2 sites, and also allowing it to bind araI site, promoting transcription. Promoter activity is also influenced by glucose levels: in its absence cAMP levels are high and the the complex cAMP+CAP (catabolite activator protein) binds to lacI promoting transcription. In presence of glucose, cAMP levels are low and promoter activity is inhibited.
Sequence and Features No part name specified with partinfo tag.