Difference between revisions of "Part:BBa J100080"

 
 
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<partinfo>BBa_J100080 short</partinfo>
 
<partinfo>BBa_J100080 short</partinfo>
  
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This part combines Riboswitch E (Part J100066) and superfolder GFP optimized for E. coli (Part J100070).
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This part combines Riboswitch E ([https://parts.igem.org/Part:BBa_J100066 Part J100066]) and superfolder GFP optimized for E. coli ([https://parts.igem.org/Part:BBa_J10070 Part J100070]). It was assembled using the Golden Gate assembly Method and the BsaI sites that were engineered into the riboswitch and the superfolder GFP.
  
 
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Latest revision as of 15:31, 19 July 2012

Riboswitch and GFP

This part combines Riboswitch E (Part J100066) and superfolder GFP optimized for E. coli (Part J100070). It was assembled using the Golden Gate assembly Method and the BsaI sites that were engineered into the riboswitch and the superfolder GFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 100
    Illegal AgeI site found at 187
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 175