Difference between revisions of "Part:BBa J100085"

Revision as of 14:58, 13 July 2012

short CRISPR sequence with GFP target spacer

The CRISPR system (Clustered Regularly Interspaced Short Palindromic Repeats) has been shown to act as a bacterial immune system. This system is characterized by short repeats, called CRISPR repeats, and in between these repeats are short, unique segments of DNA that have been acquired from foreign DNA that particular bacteria or archaea has encountered. E.coli, as well as other bacteria and archaea can use these "target spacer" segments to attack and destroy the foreign objects that contain that DNA, thereby functioning as an immune system. This part is an engineered synthetic CRISPR system to be used in E.coli. The one target spacer is a short, 30 bp sequence that targets the GFP gene (part E0040).

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal suffix found in sequence at 220
Illegal XbaI site found at 15
12
INCOMPATIBLE WITH RFC[12]
Illegal SpeI site found at 221
Illegal PstI site found at 235
Illegal NotI site found at 6
Illegal NotI site found at 228
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 214
23
INCOMPATIBLE WITH RFC[23]
Illegal suffix found in sequence at 221
Illegal XbaI site found at 15
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 15
Illegal SpeI site found at 221
Illegal PstI site found at 235
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_J100085 short</partinfo>
-The CRISPR system (Clustered Regularly Interspaced Short Palindromic Repeats) has been shown to act as a bacterial immune system. This system is characterized by short repeats, called CRISPR repeats, and in between these repeats are short, unique segments of DNA that have been acquired from foreign DNA that particular bacteria or archaea has encountered. E.coli, as well as other bacteria and archaea can use these "target spacer" segments to attack and destroy the foreign objects that contain that DNA, thereby functioning as an immune system. This part is an engineered synthetic CRISPR system to be used in E.coli. It includes a short 30 bp sequence that targets the GFP gene (E0040).
+The CRISPR system (Clustered Regularly Interspaced Short Palindromic Repeats) has been shown to act as a bacterial immune system. This system is characterized by short repeats, called CRISPR repeats, and in between these repeats are short, unique segments of DNA that have been acquired from foreign DNA that particular bacteria or archaea has encountered. E.coli, as well as other bacteria and archaea can use these "target spacer" segments to attack and destroy the foreign objects that contain that DNA, thereby functioning as an immune system. This part is an engineered synthetic CRISPR system to be used in E.coli. The one target spacer is a short, 30 bp sequence that targets the GFP gene (part E0040).
 <!-- Add more about the biology of this part here