Difference between revisions of "Part:BBa J100071"
 
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<partinfo>BBa_J100071 short</partinfo>
 
<partinfo>BBa_J100071 short</partinfo>
  
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The promoter for the cadBA operon in ''E. coli''. It is induced by low pH ([http://ecocyc.org/ECOLI/NEW-IMAGE?type=GENE-IN-MAP&object=EG10131 cadA gene]). CadC senses external pH, by low pH preventing the formation of a disulfide bond, activating CadC. When activated, CadC binds to two sites upstream of the cadBA promoter, Cad1 and Cad2. The binding of CadC to these sites activates transcription by releasing the H-NS molecules that are bound to, and repressing, the cadBA operon ([http://ecocyc.org/ECOLI/NEW-IMAGE?type=ENZYME&object=PD00436 CadC function in cadBA operon]). Cloned into pSB1A8.
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The promoter for the cadBA operon in ''E. coli''.  
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Induced by low extracellular pH ([http://ecocyc.org/ECOLI/NEW-IMAGE?type=GENE-IN-MAP&object=EG10131 cadA gene]).  
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CadC senses external pH, by low pH preventing the formation of a disulfide bond, activating CadC. When activated, CadC binds to two sites upstream of the cadBA promoter, Cad1 and Cad2. The binding of CadC to these sites activates transcription by releasing the H-NS molecules that are bound to, and repressing, the cadBA operon ([http://ecocyc.org/ECOLI/NEW-IMAGE?type=ENZYME&object=PD00436 CadC function in cadBA operon]).  
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Cloned into pSB1A8.
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Similar to [https://parts.igem.org/wiki/index.php/Part:BBa_K112405 K112405].
  
 
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Latest revision as of 21:54, 5 July 2012

cadA promoter

The promoter for the cadBA operon in E. coli.

Induced by low extracellular pH ([http://ecocyc.org/ECOLI/NEW-IMAGE?type=GENE-IN-MAP&object=EG10131 cadA gene]).

CadC senses external pH, by low pH preventing the formation of a disulfide bond, activating CadC. When activated, CadC binds to two sites upstream of the cadBA promoter, Cad1 and Cad2. The binding of CadC to these sites activates transcription by releasing the H-NS molecules that are bound to, and repressing, the cadBA operon ([http://ecocyc.org/ECOLI/NEW-IMAGE?type=ENZYME&object=PD00436 CadC function in cadBA operon]).

Cloned into pSB1A8.

Similar to K112405.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]