Difference between revisions of "Part:BBa K917001:Experience"

 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
Line 5: Line 4:
  
 
===Applications of BBa_K917001===
 
===Applications of BBa_K917001===
 +
Expression of this BioBrick allows growth of E. coli K12-derived strains with sucrose as sole carbon source in presence of sodium arsenate. We (Edinburgh 2012) have tested this BioBrick by growing transformed E. coli on range of different media; Results below were obtained after 4 days of incubation at 37 degrees Celcius. Transformed cells managed to from colonies on plate with sucrose added as sole carbon source, whereas vector control cells failed to grow.
 +
 +
<gallery widths=200px heights=200px style="border:none; text-align:center;background: white;">
 +
Image:Cml_DNA_(-).jpg|Plate with chloramphenicol; untransformed cells
 +
Image:Cml_DNA_(+).jpg|Plate with chloramphenicol; transformed cells
 +
Image:GLU DNA (-).jpg|Plate with glucose as carbon source; untransformed cells
 +
Image:GLU_DNA_(+).jpg|Plate with glucose as carbon source; transformed cells
 +
Image:SUC_DNA_(-).jpg|Plate with sucrose as carbon source; untransformed cells
 +
Image:SUC_DNA_(+).jpg|Plate with sucrose as carbon source; transformed cells
 +
Image:No_S_DNA_(-).jpg|Control plate with no sugars; untransformed cells
 +
Image:No_S_DNA_(+).jpg|Control plate with no sugars; transformed cells
 +
</gallery>
 +
  
 
===User Reviews===
 
===User Reviews===

Revision as of 09:40, 3 July 2012

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K917001

Expression of this BioBrick allows growth of E. coli K12-derived strains with sucrose as sole carbon source in presence of sodium arsenate. We (Edinburgh 2012) have tested this BioBrick by growing transformed E. coli on range of different media; Results below were obtained after 4 days of incubation at 37 degrees Celcius. Transformed cells managed to from colonies on plate with sucrose added as sole carbon source, whereas vector control cells failed to grow.

  • Plate with chloramphenicol; untransformed cells

  • Plate with chloramphenicol; transformed cells

  • Plate with glucose as carbon source; untransformed cells

  • Plate with glucose as carbon source; transformed cells

  • Plate with sucrose as carbon source; untransformed cells

  • Plate with sucrose as carbon source; transformed cells

  • Control plate with no sugars; untransformed cells

  • Control plate with no sugars; transformed cells


User Reviews

UNIQf07d1a01a4bb659a-partinfo-00000001-QINU UNIQf07d1a01a4bb659a-partinfo-00000002-QINU