Difference between revisions of "Part:BBa K862000"
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===Characterisation===
 
===Characterisation===
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This part was characterized during the 2012 HS competition by the Heidelberg LSL team
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This part was characterized during the 2012 HS competition by the Heidelberg_LSL team
 
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<center><img src="https://static.igem.org/mediawiki/2012hs/8/80/Bildschirmfoto_2012-06-11_um_23.47.05.png" width="500"/></center>
 
<center><img src="https://static.igem.org/mediawiki/2012hs/8/80/Bildschirmfoto_2012-06-11_um_23.47.05.png" width="500"/></center>
 
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<b>Fig. 1: Comparison of parts BBa_K862000 and BBa_K862001 via ONPG assay of Bl21(DE3) UV-irradiated for different times. </b>Both constructs show a strong correlation between the UV-irradiation time and the LacZ activity (as measured by the production of o-nitrophenol). <br/>
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<b>Fig. 1: Comparison of parts <a href="https://parts.igem.org/Part:BBa_K862000">BBa_K862000 (precA_LacZ)</a> and <a href="https://parts.igem.org/Part:BBa_K862001 (psulA_LacZ)">BBa_K862001</a> via ONPG assay of Bl21(DE3) UV-irradiated for different times. </b>Both constructs show a strong correlation between the UV-irradiation time and the LacZ activity (as measured by the production of o-nitrophenol). <br/>
 
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<center><img src="https://static.igem.org/mediawiki/2012hs/c/c7/XGAL_Measurement.PNG" width="635"/></center>
 
<center><img src="https://static.igem.org/mediawiki/2012hs/c/c7/XGAL_Measurement.PNG" width="635"/></center>
 
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<b>Fig. 2: X-gal assay of Bl21(DE3) transformed with precA/precB/psulA_LacZ parts (BBa_K862000, BBa_K862001, BBa_K862002) and irradiated for different times. </b>All constructs show a strong positive correlation between UV induction time and coloring of the wells. PrecA-LacZ (BBa_K862000) gives the lowest reporter background expression whereas psulA-LacZ (BBa_K862001) gives the highest overall coloring of the samples.<br/>
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<b>Fig. 2: X-gal assay of Bl21(DE3) transformed with <a href="https://parts.igem.org/Part:BBa_K862000">BBa_K862000</a>, <a href="https://parts.igem.org/Part:BBa_K862001">BBa_K862001</a> and <a href="https://parts.igem.org/Part:BBa_K862002">BBa_K862002</a> and irradiated for different times. </b>All constructs show a strong positive correlation between UV induction time and coloring of the wells. PrecA-LacZ (BBa_K862000) gives the lowest reporter background expression whereas psulA-LacZ (BBa_K862001) gives the highest overall coloring of the samples.<br/>
 
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Revision as of 12:25, 25 June 2012

precA-LacZ-doubleTerminator

This is a part for the precise quantification of UV-radiation or radioactive radiation in E. coli (recA+) strains, i.e. BL21(DE3). It consits of a recA Promoter (part BBa_J22106) fused to a LacZ reporter cloned in front of a double terminator (part BBa_K173004).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Characterisation

This part was characterized during the 2012 HS competition by the Heidelberg_LSL team

Fig. 1: Comparison of parts BBa_K862000 (precA_LacZ) and BBa_K862001 via ONPG assay of Bl21(DE3) UV-irradiated for different times. Both constructs show a strong correlation between the UV-irradiation time and the LacZ activity (as measured by the production of o-nitrophenol).



Fig. 2: X-gal assay of Bl21(DE3) transformed with BBa_K862000, BBa_K862001 and BBa_K862002 and irradiated for different times. All constructs show a strong positive correlation between UV induction time and coloring of the wells. PrecA-LacZ (BBa_K862000) gives the lowest reporter background expression whereas psulA-LacZ (BBa_K862001) gives the highest overall coloring of the samples.