Difference between revisions of "Part:BBa K862003"
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<partinfo>BBa_K862003 parameters</partinfo>
 
<partinfo>BBa_K862003 parameters</partinfo>
 
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===Characterization===
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This part was characterized by iGEM team Heidelberg_LSL 2012 in context of part
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<a href="https://parts.igem.org/Part:BBa_K862003" class="external text" title="https://parts.igem.org/Part:BBa_K862002" rel="nofollow">BBa_K862002</a>. Please find the characterization details on the referred parts page.

Revision as of 12:19, 25 June 2012

precB

The recB promoter sequence was taken for the E. coli MG1655 genome sequence (http://ecoliwiki.net/colipedia/index.php/recB:Gene). We assumed the main promoter region to be located from -70 to -1 bp upstream the recB start codon. Therefore this sequence was synthesized and cloned on an oligo basis.

We will not submit the physical DNA of this part, but we provide the oligo-sequences you can use for synthesizing and cloning this part in front of any EcoRI/XbaI precut reporter part.

RecB_fw: aattcgcggccgcttctagagCCTGAAGGCTGGAAAGTGTGGGAGAACGTCAGCGCGTTGCAGCAAACAATGCCCCTGATGAGTGAAAAGAc

RecB_rev: ctaggTCTTTTCACTCATCAGGGGCATTGTTTGCTGCAACGCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGctctagaagcggccgcg


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Characterization

This part was characterized by iGEM team Heidelberg_LSL 2012 in context of part <a href="https://parts.igem.org/Part:BBa_K862003" class="external text" title="https://parts.igem.org/Part:BBa_K862002" rel="nofollow">BBa_K862002</a>. Please find the characterization details on the referred parts page.