Difference between revisions of "Part:BBa K727622"
 
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GPPS (Geranyl diphosphate synthase) synthesizes GPP (Geranyl diphosphate) which is the first reaction of geraniol production.GS (Geraniol syntase) synthesizes Geraniol which is the second reaction of geraniol production. We added Lux cassette right promoter that up-regulated by HSL and LuxR in the course of being able to regulate easily. We intended to generate a positive feedback response with the help of producing LuxR and HSL and activating the promoter with them.  
 
GPPS (Geranyl diphosphate synthase) synthesizes GPP (Geranyl diphosphate) which is the first reaction of geraniol production.GS (Geraniol syntase) synthesizes Geraniol which is the second reaction of geraniol production. We added Lux cassette right promoter that up-regulated by HSL and LuxR in the course of being able to regulate easily. We intended to generate a positive feedback response with the help of producing LuxR and HSL and activating the promoter with them.  

Latest revision as of 00:20, 17 June 2012

Rose Odor Generator Device (Geraniol)-Complete System

GPPS (Geranyl diphosphate synthase) synthesizes GPP (Geranyl diphosphate) which is the first reaction of geraniol production.GS (Geraniol syntase) synthesizes Geraniol which is the second reaction of geraniol production. We added Lux cassette right promoter that up-regulated by HSL and LuxR in the course of being able to regulate easily. We intended to generate a positive feedback response with the help of producing LuxR and HSL and activating the promoter with them.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 64
    Illegal suffix found in sequence at 984
    Illegal EcoRI site found at 1013
    Illegal XbaI site found at 1028
    Illegal SpeI site found at 2843
    Illegal PstI site found at 2857
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 64
    Illegal EcoRI site found at 1013
    Illegal SpeI site found at 985
    Illegal SpeI site found at 2843
    Illegal PstI site found at 999
    Illegal PstI site found at 2857
    Illegal NotI site found at 70
    Illegal NotI site found at 992
    Illegal NotI site found at 1019
    Illegal NotI site found at 2850
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 64
    Illegal EcoRI site found at 1013
    Illegal BglII site found at 4292
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 64
    Illegal suffix found in sequence at 985
    Illegal EcoRI site found at 1013
    Illegal XbaI site found at 1028
    Illegal SpeI site found at 2843
    Illegal PstI site found at 2857
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 64
    Illegal EcoRI site found at 1013
    Illegal XbaI site found at 79
    Illegal XbaI site found at 1028
    Illegal SpeI site found at 985
    Illegal SpeI site found at 2843
    Illegal PstI site found at 999
    Illegal PstI site found at 2857
    Illegal NgoMIV site found at 341
    Illegal AgeI site found at 246
    Illegal AgeI site found at 627
    Illegal AgeI site found at 1194
  • 1000
    COMPATIBLE WITH RFC[1000]